https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 The Acinetobacter baumannii disinfectant resistance protein, AmvA, is a spermidine and spermine efflux pump https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39776 Wed 22 Jun 2022 12:26:08 AEST ]]> Increasing the protein quantity in a meal results in dose-dependent effects on postprandial glucose levels in individuals with Type 1 diabetes mellitus https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30973 Wed 19 Jan 2022 15:16:49 AEDT ]]> First-in-class inhibitor of ribosomal RNA synthesis with antimicrobial activity against Staphylococcus aureus https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32509 Staphylococcus aureus (MRSA). A pharmacophore model was constructed on the basis of the protein-protein interaction between essential bacterial rRNA transcription factors NusB and NusE and employed for an in silico screen to identify potential leads. One compound, (E)-2-{[(3-ethynylphenyl)imino] methyl}-4-nitrophenol (MC4), demonstrated antimicrobial activity against a panel of S. aureus strains, including MRSA, without significant toxicity to mammalian cells. MC4 resulted in a decrease in the rRNA level in bacteria, and the target specificity of MC4 was confirmed at the molecular level. Results obtained from this work validated the bacterial rRNA transcription machinery as a novel antimicrobial target. This approach may be extended to other factors in rRNA transcription, and MC4 could be applied as a chemical probe to dissect the relationship among MRSA infection, MRSA growth rate, and rRNA synthesis, in addition to its therapeutic potential.]]> Wed 15 Dec 2021 16:07:19 AEDT ]]> Influence of dietary protein on postprandial blood glucose levels in individuals with type 1 diabetes mellitus using intensive insulin therapy https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:24093 0.05). Protein loads of 75 and 100 g resulted in lower glycaemic excursions than control in the 60-120 min postprandial interval, but higher excursions in the 180-300 min interval. In comparison with 20 g glucose, the large protein loads resulted in significantly delayed and sustained glucose excursions, commencing at 180 min and continuing to 5 h. Conclusions: Seventy-five grams or more of protein alone significantly increases postprandial glycaemia from 3 to 5 h in people with Type 1 diabetes mellitus using intensive insulin therapy. The glycaemic profiles resulting from high protein loads differ significantly from the excursion from glucose in terms of time to peak glucose and duration of the glycaemic excursion. This research supports recommendations for insulin dosing for large amounts of protein.]]> Wed 11 Apr 2018 17:03:56 AEST ]]> Oncogenic activation of MEK/ERK primes melanoma cells for adaptation to endoplasmic reticulum stress https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14866 Wed 11 Apr 2018 16:08:27 AEST ]]> The association between the macronutient content of maternal diet and the adequacy of micronutrients during pregnancy in the Women and Their Children's Health (WATCH) Study https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:12880 Wed 11 Apr 2018 15:06:18 AEST ]]> Activation of XerCD-dif recombination by the FtsK DNA translocase https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23206 Wed 11 Apr 2018 13:59:44 AEST ]]> Protein and vegetarian diets https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19372 Wed 11 Apr 2018 09:59:48 AEST ]]> A cytosolic sperm protein factor mobilizes Ca²⁺ from intracellular stores by activating multiple Ca²⁺ release mechanisms independently of low molecular weight messengers https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:6596 Wed 11 Apr 2018 09:57:51 AEST ]]> Additional Insulin Is Required in Both the Early and Late Postprandial Periods for Meals High in Protein and Fat: A Randomized Trial https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49023 Wed 03 May 2023 12:38:05 AEST ]]> Reducing the postprandial glycaemic impact of dietary protein in type 1 diabetes mellitus https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36525 Wed 01 Jul 2020 11:21:51 AEST ]]> Lower protein-to-carbohydrate ratio in maternal diet is associated with higher childhood systolic blood pressure up to age four years https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22165 40% of energy) intakes. There may be an ideal maternal macronutrient ratio associated with optimal infant BP. Maternal diet, which is potentially modifiable, may play an important role in influencing offspring risk of future hypertension.]]> Tue 24 Apr 2018 11:39:46 AEST ]]> Plasma metabolomic response to high-carbohydrate meals of differing glycaemic load in overweight women https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50962 Tue 22 Aug 2023 11:38:37 AEST ]]> Addressing sarcopenia in an aging population https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:55572 Tue 11 Jun 2024 11:22:53 AEST ]]> Encapsulation of citrus by-product extracts by spray-drying and freeze-drying using combinations of maltodextrin with soybean protein and ι-Carrageenan https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33467 w) than those produced by spray-drying. Specifically, the MC and aw of the microparticles produced by freeze-drying ranged from 1.15 to 2.15% and 0.13 to 0.14, respectively, while the MC and aw of the microparticles produced by spray-drying ranged from 6.06% to 6.60% and 0.33 to 0.40, respectively. Scanning electron microscopy revealed that spray-drying resulted in the formation of spherical particles of different sizes regardless of the type of coating agent. Although freeze-drying resulted in microparticles with amorphous glassy shapes, the mixture of MD + soybean protein resulted in the formation of spherical porous particles. X-ray diffraction revealed a low degree of crystallinity for the samples produced by both techniques.]]> Tue 03 Sep 2019 17:59:14 AEST ]]> LncRNA REG1CP promotes tumorigenesis through an enhancer complex to recruit FANCJ helicase for REG3A transcription https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37758 regenerating islet-derived (REG) gene family are important regulators of many cellular processes. Here we functionally characterise a non-protein coding product of the family, the long noncoding RNA (lncRNA) REG1CP that is transcribed from a DNA fragment at the family locus previously thought to be a pseudogene. REG1CP forms an RNA–DNA triplex with a homopurine stretch at the distal promoter of the REG3A gene, through which the DNA helicase FANCJ is tethered to the core promoter of REG3A where it unwinds double stranded DNA and facilitates a permissive state for glucocorticoid receptor α (GRα)-mediated REG3A transcription. As such, REG1CP promotes cancer cell proliferation and tumorigenicity and its upregulation is associated with poor outcome of patients. REG1CP is also transcriptionally inducible by GRα, indicative of feedforward regulation. These results reveal the function and regulation of REG1CP and suggest that REG1CP may constitute a target for cancer treatment.]]> Thu 27 Jan 2022 15:55:02 AEDT ]]> Glial glutamate transporter expression patterns in brains from multiple mammalian species https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:625 Thu 25 Jul 2013 09:10:23 AEST ]]> Nutrition intervention improves outcomes in patients with cancer cachexia receiving chemotherapy - a pilot study (short communication) https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:5 Thu 25 Jul 2013 09:10:12 AEST ]]> Epidermal growth factor induces tyrosine phosphorylation, membrane insertion, and activation of transient receptor potential channel 4 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:494 Thu 25 Jul 2013 09:09:56 AEST ]]> Plasma Amino Acid Appearance and Status of Appetite Following a Single Meal of Red Meat or a Plant-Based Meat Analog: A Randomized Crossover Clinical Trial https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49996 0.9), although several nonessential amino acids differed strongly according to participant BMI. Postprandial amino acids peaked at 2–3 h in all groups. The BB meal produced significantly lower plasma concentrations of total, essential, branched-chain, and non-proteogenic amino acids than the Lamb, Pasture, or Grain meals, based on AUC. There were no significant differences between meal groups in scores for hunger, fullness, or cravings. Conclusions: Red meat meals exhibited greater bioavailability of amino acids compared with the PBMA (BB). Pasture versus Grain origins of the beef had little influence on participants’ responses. This trial was registered at ClinicalTrials.gov as NCT04545398.]]> Thu 22 Jun 2023 14:26:36 AEST ]]> Elevated plasma ferritin in elderly individuals with high neocortical amyloid-β load https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36161 Thu 20 Feb 2020 14:45:19 AEDT ]]> Replication fork collapse at a protein-DNA roadblock leads to fork reversal, promoted by the RecQ helicase https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34848 Thu 16 May 2019 17:38:51 AEST ]]> Novel remediation of per- and polyfluoroalkyl substances (PFASs) from contaminated groundwater using Cannabis Sativa L. (hemp) protein powder https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42691 Cannabis sativa L.) were found to be far superior for PFAS removal than the next best protein, soy. The use of hemp plant proteins as a possible pump-and-treat solution to PFAS remediation from groundwater has been successfully demonstrated with very good removals (>98%) of the main contaminants of PFOS and PFHxS in approximately 1 h of contact time, with salinity enhancing removal of short chain PFAS. Changes to the secondary structure of hemp proteins was found using FTIR spectroscopy analysis and calculated based on the integrated areas of the amide I component bands. The amount of β-turns increased from ∼9.3% (control) to 44.1% (undiluted groundwater); with a decrease in random coils (25.6–8.6%); α-helix (19.3–8.6%) and β-sheets (38.8–23.1%). These changes indicate that hemp proteins partially unfold during the reaction with PFAS with other FTIR evidence suggesting sorption at hydrophobic sites of the protein as well as with the side chains of the amino acids aspartic and glutamic acid. The absence of these side chains in soy protein, as evidenced from FTIR and amino acid analysis, being part of the reason why soy removed less (approx. half) of the Σ(PFHxS + PFOS) load when compared to hemp. The findings reported here will lead to new, environmentally friendly methods for PFAS remediation.]]> Thu 01 Sep 2022 09:43:23 AEST ]]> A central role for venom in predation by Varanus komodoensis (Komodo Dragon) and the extinct giant Varanus (Megalania) priscus https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:8224 Sat 24 Mar 2018 08:40:37 AEDT ]]> Global survey of protein expression during gonadal sex determination in mice https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:7528 Sat 24 Mar 2018 08:38:31 AEDT ]]> High-protein diets during pregnancy: healthful or harmful for offspring? https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16914 Sat 24 Mar 2018 07:58:45 AEDT ]]> Small molecular weight variants of p53 are expressed in human melanoma cells and are induced by the DNA-damaging agent cisplatin https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:5341 Sat 24 Mar 2018 07:45:56 AEDT ]]> Impact of fat, protein, and glycemic index on postprandial glucose control in type 1 diabetes: implications for intensive diabetes management in the continuous glucose monitoring era https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28288 Sat 24 Mar 2018 07:41:23 AEDT ]]> Proteotranscriptomic profiling of 231-BR breast cancer cells: identification of potential biomarkers and therapeutic targets for brain metastasis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28878 Sat 24 Mar 2018 07:40:30 AEDT ]]> Bacterial sliding clamp inhibitors that mimic the sequential binding mechanism of endogenous linear motifs. https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28373 Escherichia coli sliding clamp occurs via a sequential mechanism that involves two subsites (I and II). Here, we report the development of small-molecule inhibitors that mimic this mechanism. The compounds contain tetrahydrocarbazole moieties as "anchors" to occupy subsite I. Functional groups appended at the tetrahydrocarbazole nitrogen bind to a channel gated by the side chain of M362 and lie at the edge of subsite II. One derivative induced the formation of a new binding pocket, termed subsite III, by rearrangement of a loop adjacent to subsite I. Discovery of the extended binding area will guide further inhibitor development.]]> Sat 24 Mar 2018 07:35:58 AEDT ]]> The impact of oxidative stress on chaperone-mediated human sperm-egg interaction https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26416 Sat 24 Mar 2018 07:27:57 AEDT ]]> The role of dietary protein and fat in glycaemic control in Type 1 diabetes: implications for intensive diabetes management https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26704 Sat 24 Mar 2018 07:26:20 AEDT ]]> Molecular architecture of the 'stressosome,' a signal integration and transduction hub https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:4738 Sat 24 Mar 2018 07:21:09 AEDT ]]> Effects of Dietary Fat and Protein on Glucoregulatory Hormones in Adolescents and Young Adults with Type 1 Diabetes https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44787 Mon 24 Oct 2022 09:17:37 AEDT ]]> Shwachman-Bodian-Diamond syndrome (SBDS) protein is a direct inhibitor of protein phosphatase 2A (PP2A) activity and overexpressed in acute myeloid leukaemia https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42359 Mon 22 Aug 2022 14:08:27 AEST ]]> Amino acid distribution in blood following high-intensity interval exercise: a preliminary study https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54752 Mon 11 Mar 2024 14:53:24 AEDT ]]> Protein Intake and Physical Activity Levels as Determinants of Sarcopenia Risk in Community-Dwelling Older Adults https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:55458 Mon 03 Jun 2024 08:51:54 AEST ]]> A Simple and Sensitive LC-MS/MS Method for the Simultaneous Determination of Cyclophosphamide and Dexorubicin Concentrations in Human Plasma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45477 Fri 28 Oct 2022 14:36:16 AEDT ]]> Cold Shock Domain Containing E1 (CSDE1) Protein is Overexpressed and Can be Targeted to Inhibit Invasiveness in Pancreatic Cancer Cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41096 Fri 22 Jul 2022 18:07:18 AEST ]]> Protein chimerization: a new frontier for engineering protein therapeutics with improved pharmacokinetics https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41754 Fri 12 Aug 2022 11:35:23 AEST ]]> Dietary protein affects both the dose and pattern of insulin delivery required to achieve postprandial euglycaemia in Type 1 diabetes: a randomized trial https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45562 P =0.001], with inter‐individual requirements ranging from 0.9 to six times the low‐protein meal requirement. Approximately half the additional insulin [1.1 units/h (CI 0.5, 1.8; P =0.001)] was given in the first 2 h, compared with an additional 0.5 units/h (CI –0.2, 1.2; P =0.148) in the second 2 h and 0.1 units (CI –0.6, 0.8; P =0.769) in the final hour. Conclusions: A high‐protein meal requires ~50% more insulin to maintain euglycaemia than a low‐protein meal that contains the same quantity of carbohydrate. The majority is required within the first 2 h. Inter‐individual differences exist in insulin requirements for dietary protein.]]> Fri 04 Nov 2022 14:45:18 AEDT ]]>